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Engineering of extracellular vesicles for small molecule-regulated cargo loading and cytoplasmic delivery of bioactive proteins
Rapamycin-dependent protein cargo sorting into extracellular vesicles and functional delivery
Masaharu Somiya, Shun’ichi Kuroda
DOI
Engineering of extracellular vesicles for small molecule-regulated cargo loading and cytoplasmic delivery of bioactive proteins
Reporter gene assay for membrane fusion of extracellular vesicles
Our preprint has been finally published in Journal of Extracellular Vesicles! We describe a novel membrane fusion assay to measure the intracellular membrane fusion of extracellular vesicles mediated by virus-derived fusion protein VSV-G.
Masaharu Somiya, Shun’ichi Kuroda
DOI
Reporter gene assay for membrane fusion of extracellular vesicles
Real-Time Luminescence Assay for Cytoplasmic Cargo Delivery of Extracellular Vesicles
We developed a novel luminescence assay to quantitatively assess the intracellular protein delivery of extracellular vesicles.
Masaharu Somiya, Shun’ichi Kuroda
DOI
Real-Time Luminescence Assay for Cytoplasmic Cargo Delivery of Extracellular Vesicles
Cytoplasmic delivery of small interfering RNA by photoresponsive non-cationic liposomes
The combination of non-cationic liposomes and photosensitizer AlPcS2a enables cytoplasmic siRNA delivery upon red light irradiation
Masaharu Somiya, Kanako Sakaeda, Yuta Ishii, Shun’ichi Kuroda
DOI
Biocompatibility of highly purified bovine milk-derived extracellular vesicles
Extracellular vesicles (EVs) deliver biologically active cargos from donor cells to recipient cells for intercellular communication. Since the existence of RNA cargo was discovered, EVs have been considered to be useful drug-delivery systems.
Masaharu Somiya, Yusuke Yoshioka, Takahiro Ochiya
DOI